狡兔死 走狗烹

The inhibition of the malignant potential by targeting tumor cell-derived exosomes in oral squamous carcinoma cells

The inhibition of the malignant potential by targeting tumor cell-derived exosomes in oral squamous carcinoma cells

山本哲也(Tetsuya YAMAMOTO)

Professor and Chairman, Department of Oral and Maxillofacial Surgery, Kochi Medical School, Kochi University

Exosomes are 30-100 nm-sized membranous vesicles, secreted from a variety of cell types into their surrounding extracellular space. Various exosome components including lipids, proteins, and nucleic acids are transferred to recipient cells and affect their function and activity. Numerous studies have showed that tumor cell-derived exosomes play important roles in tumor growth and progression. However, the effect of exosomes released from oral squamous cell carcinoma (OSCC) into the tumor microenvironment remains unclear. In the present study, we isolated exosomes from OSCC cells and investigated the influence of OSCC cell-derived exosomes on the tumor cell behavior associated with tumor development. We demonstrated that OSCC cell- derived exosomes were taken up by OSCC cells themselves and significantly promoted proliferation, migration, and invasion through the activation of the PI3K/Akt, MAPK/ERK, and JNK-1/2 pathways in vitro. These effects of OSCC cell-derived exosomes were obviously attenuated by treatment with PI3K, ERK-1/2, and JNK- 1/2 pharmacological inhibitors. Furthermore, the growth rate of tumor xenografts implanted into nude mice was promoted by treatment with OSCC cell-derived exosomes. The uptake of exosomes by OSCC cells and subsequent tumor progression was abrogated in the presence of heparin. Taken together, these data suggest that OSCC cell-derived exosomes might be a novel therapeutic target and the use of heparin to inhibit the uptake of OSCC-derived exosomes by OSCC cells may be useful for treatment.

Curriculum Vitae

1979-1985 Tokushima University Faculty of Dentistry
1985- Kochi Medical School Hospital
1986- Otsuki Hospital
1987- Instructor, Kochi Medical School Hospital
1993- Senior Lecturer, Kochi Medical School Hospital
2000- Associate Professor, Kochi Medical School Hospital
2002-2003 Visiting Scientist, Medical College of Georgia, USA
2004- Professor and Chairman, Kochi Medical School, Kochi University

Two jaw surgery using CAD/CAM wafer based on soft tissue 3D simulation

Two jaw surgery using CAD/CAM wafer based on soft tissue 3D simulation

富永和宏(Kazuhiro TOMINAGA) 九州歯科大学 Kyushu Dental University The goal of orthognathic surgery is to harmonize optimal aesthetic features with functional occlusion. To achieve this goal, precise morphological and functional analyses as well as optimal planning of the final goal during pre-surgical treatment is mandatory. Additionally, accurate reproduction of the simulated goal during operation is necessary. Conventional pre-surgical planning has been made with 2D cephalometric prediction. However, to achieve optimum aesthetic and occlusal results, 3D planning is going to be necessary. Especially in facial asymmetry cases which need surgical correction of not only pitch and roll but yaw, 3D simulation is mandatory. In this paper, I'm going to present two jaw surgery using intermediate wafer fabricated by CAD/ CAM based on soft tissue 3D simulation in facial asymmetry cases. Gross planning mainly relating the surgical correction of pitch and roll of the maxilla is followed by 2D analyses. The data are introduced to 3D virtual surgery program (SimPlant Pro OMS 10.1; Materialise Leuven, Belgium). Soft tissue surface reconstruction after virtual surgery is visually evaluated by some orthodontists and oral surgeons. Then little by little correction of yaw in the software is tested maintaining facial midline. The repositioning position of the maxilla in the best aesthetic feature judged by evaluators is the final goal of the operation. In this position of the maxilla, intermediate wafer is fabricated using CAD/CAM. This method can eliminate many time-consuming laboratory steps. Additionally it can reduce technical errors derived from manual preparation ie, face bow transfer, manual reposition of the plaster cast etc. I will present the accuracy of the method comparing virtual surgery and CT taken after the operation.

Osteosarcoma of the jaws in Koreans: analysis of 26 cases

Osteosarcoma of the jaws in Koreans: analysis of 26 cases 金亨埈(Hyung-Jun KIM) Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry, Seoul, Korea Objective: In order to assess clinical behavior, response to treatment, and factors influencing prognosis of patients with osteosarcoma of the jaws (OSJ) in Koreans. Methods: A retrospective study of clinical, pathological, and histological records was undertaken for 26 patients with OSJ treated at the department of Oral and maxillofacial surgery in Yonsei University Dental Hospital from 1990 to March, 2016. Result: Out of 26 patients studies, there were 9 men (34.6%) and 17 women (65.4%). Twentyone of 26 patients had osteosarcoma of the mandible, and 5 of 26 patients had osteosarcoma of maxilla. The histopathology of OSJ is highly variable, ranging from chondroblastic type (6 patients out of 26), osteoblastic type (10 patients out of 26), fibroblastic type (2 patients out of 26), to the rare variants like mixed type and small cell osteoscarcoma types among others. All patients underwent gross total excision and only few patients underwent the neoadjuvant chemotherapy. Postoperative chemotherapy was given to most of the patient as adjuvant or in combination with radiotherapy. The overall survival rate was 73.1% with a overall 2-yeaer survival rate of 83.3%. The overall 5-,10-,15-year survival rates in this study were 73.5%, 73.5%, 49%, respectively. Using Kaplan-Meier analysis with log rank tests, the size of tumor (T-stage), and resection margins were found to affect the survival rate significantly. The chemotherapy was not significantly associated with improved survival rate. Conclusion: Surgical resection with clear margin is the most important factor in disease survival. The role for chemotherapy and radiotherapy in osteosarcoma of the jaws remains controversial, which deserves further studies. Curriculum Vitae Graduated from Yonsei University College of Dentistry and took one year Internship and three year Residency, and two year Fellowship in Oral and Maxillofacial Surgery at Yonsei University Health System. Received Doctor of Dental Surgery and Master of Science in Dentistry degrees in Yonsei University and Dr. med. dent. degree in Ludwig-Maximilians University, Munich, Germany. Visiting Scholar, Oral and Maxillofacial Surgery, Ludwig-Maximilians University, Munich, Germany. (Oct. 1997 - Sep. 1999)–6–

Visiting Professor, Institute of Surgical Experiment, Ludwig-Maximilians University, Munich, Germany. (Feb. 2006 - Jan. 2007) Visiting Professor, Oral and Maxillofacial Surgery, Tokyo Dental College, Japan. (Jun. 2008) Visiting Professor, Oral and Maxillofacial Surgery, Technical University of Munich, Germany. (Oct. 2013) Professor and Chairman in Oral and Maxillofacial Surgery, Yonsei University College of Dentistry. Adjunct Professor in Oral Biology, Yonsei University College of Dentistry Director of Education and Research, Yonsei University Dental Hospital Research Ambassador, German Academic Exchange Service Secretary General, Korean Association of Oral and Maxillofacial Surgeons

My Modified Surgical Tips from Original Orthognathic Procedures -Experience-based Suggestion

My Modified Surgical Tips from Original Orthognathic Procedures -Experience-based Suggestion

金 容德教授　(Professor Yong-Deok KIM)

Department of Oral and Maxillofacial Surgery, School of Dentistry,

Pusan National University, KOREA

Orthognathic surgery is procedure designated to correct conditions of the jaw and face related to structure, growth, sleep apnea, TMJ disorders, malocclusion problems owing to skeletal disharmonies or other orthodontic needs. It has been well documented in the numerous texts and very familiar with recent oral and maxillofacial surgeons all around the world. Each step of the procedure is clearly noticed with proper reason and purposes and guaranty the safe surgery. But some surgeons might modify any step for their own object under their experiences and sometimes the modified step was very useful, reliable and reasonable even they were not commented in any text or article. The present presentation would introduce and suggest my tips which were used practically during my orthognathic surgery. The following modified procedures are part of my tips which are going to mention to the audience.

1. Out-fracture of inferior turbinate
2. Intentional fracture of pterygoid plate
3. Swing cinch
4. Effective exposure of descending palatine artery

I don't think those procedures are evidence-based but hope they might be the useful idea or applied to the audience's daily surgery.

Curriculum Vitae
Professor, Dept. of OMFS, School of Dentistry, Pusan National University, KOREA
Head, Dept. of OMFS, Pusan National University Dental Hospital
Director, Institute of Translational Dental Sciences, Pusan National University, KOREA
Director, Institutional Review Board(IRB) of Pusan National University Dental Hospital
Fellow, International Team for Implantology(ITI)
(former) Visiting Scholar, Dept. of 1st OMFS, School of Dentistry, Kyushu University, JAPAN
(former) Postdoctoral Fellow, Dept. of OMFS, Faculty of Dentistry, Univ. of Toronto, CANADA

會員大會Assembly

 17:10 - 17:40 

International Convention Center

Chorus KMU Singers

16:40 - 17:10

高雄醫學大學 聲樂社

Kaohsiung Medical University Singers

陳俊志醫師指揮

Conducted by Dr William Chun-Chih CHEN, DDS

曲目

童年       Childhood        子供時代

苦行的路 Ascentic Road アスケチックロード

台灣        Taiwan            台湾